MICRO CRYOBANK - CRYOVIALS WITH BEADS

MICRO CRYOBANK is a simple method for long-term storage and retrieval of microbial cultures

MICRO CRYOBANK - store 25 identical cultures in one cryovial

MICRO CRYOBANK is a simple method for maintenance of microbial cultures. The system consists of a vial with 25 beads to which microorganisms can adhere. The beads are covered in a special cryopreservative solution.

MICRO CRYOBANK allows you to store 25 identical microbial cultures in just one vial saving space in the freezer. The system allows you to remove individual beads without thawing the rest and thus avoid damage from repeated freeze-thaw cycles.

The system gives you the opportunity to obtain a cryobank of your microorganisms at -80℃ for many years. Each vial has an individual unique barcode and a writing area.

MAXXLINE 9x9 cryo box

MICRO CRYOBANK benefits

Multiple subcultures

  • Create and store multiple subcultures within minutes.
  • Fresh culture each time

  • It is very simple to retrieve bacterial strains which provides you with a fresh culture each time.
  • Identical cultures

  • Remove individual beads without thawing the rest and avoid damage from repeated freeze-thaw cycles.
  • Unique barcode

  • Supplied in freezer storage boxes each vial has a unique printed barcode and writing space allowing easy indexing and location of cultures.
  • Long-term storage

  • Obtain a cryobank of microorganisms for longterm freezing.
  • Save space

  • Store 25 identical microbial cultures in one vial - saves a lot of space in the freezer.
  • Storage of microorganism

    For most optimal recovery of the microorganism, pick off enough colonies to achieve a 3-4 McFarland standard in the cryopreservative solution. An overnight culture (18-24 hours) is preferred.
    All procedures should be performed with aseptic techniques.

    MAXXLINE 10x10 Cryo Box MAXXLINE 10x10 Cryo Box



    1) Open the cryovial. Use a sterile inoculating loop or cotton swab to transfer colonies to the solution.

    MAXXLINE 10x10 Cryo Box MAXXLINE 10x10 Cryo Box

    2) Close the cryovial and invert it 4-5 times to emulsify. Do NOT vortex!

    MAXXLINE 10x10 Cryo Box MAXXLINE 10x10 Cryo Box

    3) Allow the cryovial to sit for about 2 minutes for adherence of the isolate to the beads.
    4) Open the vial and aspirate as much of the cryopreservative solution as possible.

    MAXXLINE 10x10 Cryo Box MAXXLINE 10x10 Cryo Box

    5) Close the cryovial finger tight only. It is important that it is not overtightened.
    6) Place the cryovial in the freezer at -80℃.

    Recovery of microorganism

    All procedures should be performed with aseptic techniques.

    MAXXLINE 10x10 Cryo Box MAXXLINE 10x10 Cryo Box

    1) Remove the cryovial from the freezer. Do not allow the vial to thaw. Placing it in a cold cryoblock extends the thawing time.
    2) Open the vial and use aseptic technique to remove one bead with sterile needle or forceps.
    3) Close the cryovial finger tight only and return it to the freezer as soon as possible.
    4) The bead can then be streaked directly onto a solid medium or it can be inoculated into liquid medium.

    Price and packaging

    You can order the vials with beads just as it suits you and your projects. The vials can be packed in 5x5, 9x9x or 10x10 cryoboxes. The vials are 2 ml and comes in 5 different colours. The 9x9 and 10x10 boxes are also available in 5 colours.

    The price per vial with 25 beads is 9.50 DKK or 1.27 euro.

    MAXXLINE 10x10 Cryo Box MAXXLINE 10x10 Cryo Box

    Are you interested in more information about this product? Please call us for a non-committal chat.